Postdoctoral Research – the role(s) of sEVs produced in the human alveolar epithelium

22 May 2023
Job Information

Organisation/Company

Université Paris Est Créteil

Department

DRV

Research Field

Biological sciences » Biology

Researcher Profile

Recognised Researcher (R2)

Country

France

Application Deadline

25 Jun 2023 – 23:59 (Europe/Brussels)

Type of Contract

Temporary

Job Status

Full-time

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

We are looking for a post-doctoral researcher whose objective will be to characterize and determine the role(s) of sEVs produced in the human alveolar epithelium. The candidate will be in charge of the purification, characterization of sEVs and the infection of cells by different variants of SARS-CoV-2 in the presence or absence of sEVs.The rapid emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in December 2019 in Wuhan, Hubei Province, China has resulted in a global pandemic. This virus is the agent responsible for the coronavirus disease 2019 (COVID-19) that has caused 688,010,809 cases and 6,872,321 deaths worldwide to date (May 2023). SARS-CoV-2 targets the human respiratory tract, which is lined with two distinct types of epithelium: the nasal epithelium of the upper respiratory tract and the alveolar epithelium of the distal airways. We investigate the role(s) of small extracellular vesicles (sEVs) produced by these epithelia during COVID-19 infection. We have elucidated the protein composition of sEVs (mu-sEVs) produced at the apical pole of uninfected human nasal epithelial cells and demonstrated that the role of mu-sEVs is to facilitate viral entry through the TMPRSS2-dependent “early pathway”, thereby promoting the infection of TMPRSS2-expressing cells.

The rapid emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in December 2019 in Wuhan, Hubei Province, China has resulted in a global pandemic. This virus is the agent responsible for the coronavirus disease 2019 (COVID-19) that has caused 688,010,809 cases and 6,872,321 deaths worldwide to date (May 2023). SARS-CoV-2 targets the human respiratory tract, which is lined with two distinct types of epithelium: the nasal epithelium of the upper respiratory tract and the alveolar epithelium of the distal airways. We investigate the role(s) of small extracellular vesicles (sEVs) produced by these epithelia during COVID-19 infection. We have elucidated the protein composition of sEVs (mu-sEVs) produced at the apical pole of uninfected human nasal epithelial cells and demonstrated that the role of mu-sEVs is to facilitate viral entry through the TMPRSS2-dependent “early pathway”, thereby promoting the infection of TMPRSS2-expressing cells

The work will be performed between the TRePCa laboratory and the IMRB team 18 (same site). The TRePCa laboratory is located at the Faculty of Health of Créteil and works on the field of extracellular vesicles in different contexts. Team 18 of the IMRB is specialized in the study of respiratory viruses. The candidate will be supervised by Dr Virginie Firlej and Dr Patrice Bruscella.

Requirements

Research Field

Biological sciences » Biology

Education Level

Master Degree or equivalent

Skills/Qualifications

The candidate must be highly motivated, preferably with a PhD in cell biology. Skills in biochemistry and molecular biology are required  (Western-blot, RT-qPCR, microscopy…). Knowledge in the field of extracellular vesicles and/or virology will be appreciated. The candidate must be able to work between two laboratories.

Research Field

Biological sciences » Biology

Internal Application form(s) needed
fiche poste post-doc TREPCA EN.pdf
English
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Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

Université Paris Est Créteil

Country

France

City

Créteil

Postal Code

94010

Street

61, avenue du Général de Gaulle

Geofield

Where to apply

E-mail

[email protected]

Contact

State/Province

France

City

Créteil

Website

http://www.u-pec.fr/

Street

61 avenue du Général de Gaulle

Postal Code

94010

E-Mail

[email protected]

Phone

33145177108

STATUS: EXPIRED