Postdoctoral researcher in plant molecular and developmental epigenetics (M/F)

Job title:

Postdoctoral researcher in plant molecular and developmental epigenetics (M/F)

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Job description

Offer DescriptionThe postdoctoral project is part of the ChromSwitch project funded by the ANR, which aims to study a function that releases Polycomb PRC2-mediated chromatin repression of developmental genes in plants.
The main objectives of the postdoctoral project are (1) to characterize further a PRC2-associated factor, carrying this switch function, including its molecular features; and (2) to resolve its interaction and opposing mechanism with PRC2.
For this, the researcher’s mission will be to develop molecular and plant genetic approaches and tool for epigenomics/epigenetics and interactomics analyses.The post-doctoral researcher will develop a project under direct supervision of C. Carles (team leader) and G. Vachon (researcher in the team). The post-doc will benefit from the team’s network of collaborations in Grenoble, within the ChromSwitch ANR consortium. In particular, the researcher will work in close interaction with the permanent engineer in charge of molecular and plant work (M. Le Masson), as well as our partner at the Institute of Structural Biology (IBS).Activities will include:

• Genome-wide profiling of epigenetic marks and proteins: ChIP-seq, Bisulfite-seq
• in vitro Protein-DNA interaction analyses: EMSA, fluorescence anisotropy, Surface Plasmon Resonance
• in vivo functional assays: Luciferase assays (protoplasts), mutant complementation analyses (Arabidopsis thaliana)
• Interactomics: IP-MS, ChIP-MS, Split-BioID-MS; Y2H, BiFC or Split-Luciferase
• in cyto immuno-localization

In addition, the post-doc will present data in English at team meetings and laboratory seminars, as well as participate to the analysis of scientific articles related to the researcher’s research, and discuss them with team members (e.g. in journal clubs).The researcher will be part of the ChromDev team ( ) of the Cellular and Plant Physiology Physiology Laboratory (UMR 5168 – CNRS -CEA – Univ. Grenoble Alpes – INRAE) in Grenoble. The team’s projects aim to better understand the Chromatin Dynamics that regulate Developmental Transitions in plants, using a multi-disciplinary strategy, encompassing in vivo phenotypic analyses, omics studies (epigenomics, transcriptomics, interactomics) and in vitro characterisation of molecular interactions and 3D structures of protein complexes.This position will take place on the Polygone campus in Grenoble, a rich environment for biology research, close to several laboratories such as IBS, IRIG, ESRF and EMBL. The LPCV institute employs around a hundred people and boasts state-of-the-art equipment for molecular biology, biochemistry, structural biology and imaging. It has plant culture facilities where all steps of molecular genetics on Arabidopsis can be carried out. The ChromDev group comprises around a dozen of members (permanent, young researchers, interns), including geneticists, molecular biologists and biochemists.RequirementsResearch Field Biological sciences Education Level PhD or equivalentResearch Field Biological sciences Education Level PhD or equivalentResearch Field Technology Education Level PhD or equivalentLanguages FRENCH Level BasicResearch Field Biological sciences » Botany Years of Research Experience 1 – 4Research Field Biological sciences Years of Research Experience 1 – 4Research Field Technology » Biotechnology Years of Research Experience 1 – 4Additional InformationEligibility criteriaThe candidate should have a profile adequate to develop molecular and plant genetic approaches for epigenomics/epigenetics and interactomic analyses. The candidate should :
– master the basics (theory and practice) of genetics/epigenetics and molecular biology.
– be familiar with methods for analyzing and measuring gene expression and epigenetic marks, and their use in bioinformatics.
be familiar with methods for bioinformatics analyses and measurements of gene expression and epigenetic marks.
– be autonomous in designing experimental plans, interpreting and presenting results at team meetings and seminars
– develop tools and methods and use them in a reliable and reproducible manner.
– be able to contribute to the preparation of scientific publications.
– be able to work independently in an interdisciplinary and international environment.
– be willing to interact scientifically in a dynamic research group.
– respect laboratory rules and procedures.
– speak fluent English.Additional comments12-month contract, renewable up to 36 months Website for additional job detailsWork Location(s)Number of offers available 1 Company/Institute Laboratoire de physiologie cellulaire et végétale Country France City GRENOBLE GeofieldWhere to apply WebsiteContact CityGRENOBLE WebsiteSTATUS: EXPIRED

Expected salary

Location

Grenoble, Isère

Job date

Fri, 26 Apr 2024 00:53:43 GMT

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