In-vivo flow NMR lipidomics – Development of innovative NMR methods for lipid profiling of whole-cell microalgae

Organisation/Company

Université de Nantes

Research Field

Chemistry
Biological sciences » Biology
Physics

Researcher Profile

Recognised Researcher (R2)
Leading Researcher (R4)
First Stage Researcher (R1)
Established Researcher (R3)

Country

France

Application Deadline

29 Apr 2024 – 22:00 (UTC)

Type of Contract

Temporary

Job Status

Full-time

Offer Starting Date

10 Jun 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Context: Microalgae are a source of biomass rich in various high value-added compounds such as lipids (mainly fatty acid triglycerides), carbohydrates, vitamins and proteins, with industrial applications ranging from food to health and energy. In this context, the project aims to develop the potential of Nuclear Magnetic Resonance (NMR) for on-line monitoring of the biochemical quality of microalgae. Among these, spirulina or Limnospira platensis (LP) is recognized for its nutritional properties as superfood. Moreover, it will be possible to offer this on-board method, which will not only control the physiological condition of these life support systems, but also their edibility.

The CEISAM and GEPEA laboratories at Nantes University have been collaborating for over 6 years now. As part of the AMER-METAL project, they demonstrated that NMR can be used to precisely determine the identity and quantity of microalgal lipids [1], directly on whole biomass, using high-field magnetic instruments. Investigations were also carried out into the capabilities of compact, lower-field NMR instruments [2] for monitoring the production of microalgae lipids.

Objectives: The main aim the project is to develop a miniaturized system based on compact NMR for on-line monitoring of high-nutrient metabolites from microalgae grown in photobioreactors. Improving the analytical performance of compact NMR systems is essential for better on-line simultaneous analysis of several families of metabolites: LP lipids. With this in mind, the PhD student’s mission will be to characterize the diversity of nutritive metabolites produced by LP using high-field NMR (700 MHz at CEISAM in Nantes), identifying lipids. Firstly, LP extracts will be studied, but also whole cells in vivo, which represents a real challenge. In this context, we will implement advanced methodologies to make signals more resolute, sensitive and accelerate their acquisition, but also capable of overcoming intracellular inhomogeneities. The high repeatability of NMR makes it a tool of choice for quantifying metabolites identified in mixtures in culture media and cells. Compact 80 MHz devices (available at CEISAM and GEPEA sites in St Nazaire) will also be evaluated with advanced methods in a second phase to validate their performance in identifying and quantifying LP lipids, compared with information obtained at 700 MHz. Finally, the coupling with a photobioreactor will be set up to monitor on-line and in real time the accumulation of metabolites of high nutritional interest in LP cells under cultivation.

References:

[1] Dylan Bouillaud, these de doctorat, 2017-2020, Multiscale NMR analysis of the microalgae metabolism

[2] Castaing-Cordier, T.; Bouillaud, D.; Farjon, J.; Giraudeau, P. Recent Advances in Benchtop NMR Spectroscopy and Its Applications. In Annual Reports on NMR Spectroscopy; Academic Press, 2021. https://doi.org/10.1016/bs.arnmr.2021.02.003 .

[3] Bouillaud, D.; Heredia, V.; Castaing-Cordier, T.; Drouin, D.; Charrier, B.; Gonçalves, O.; Farjon, J.; Giraudeau, P. Benchtop Flow NMR Spectroscopy as an Online Device for the in Vivo Monitoring of Lipid Accumulation in Microalgae. Algal Research 2019, 43, 101624. https://doi.org/10.1016/j.algal.2019.101624 .

[4] Bouillaud, D.; Drouin, D.; Charrier, B.; Jacquemmoz, C.; Farjon, J.; Giraudeau, P.; Gonçalves, O. Using Benchtop NMR Spectroscopy as an Online Non-Invasive in Vivo Lipid Sensor for Microalgae Cultivated in Photobioreactors. Process Biochemistry 2020, 93, 63–68. https://doi.org/10.1016/j.procbio.2020.03.016 .

Funding category: Financement public/privé

PHD title: Doctorat de Chimie Analytique
PHD Country: France

Requirements
Specific Requirements

Profile: Candidates must have an Engineer or a Master's degree in (Bio)Analytical Chemistry or Physical Chemistry and have a solid theoretical knowledge in NMR. Previous experience in this field (internship) is appreciated but not essential. The engineer will also need to be open-minded, given the interdisciplinary and collaborative nature of the project, carried out between two laboratories in the Nantes area. This recruitment will offer a unique opportunity to an engineer or a master 2 student to invest in innovative developments in high and low field NMR, at the interface between spectroscopy, analytical chemistry, biology and process engineering, to a better understanding of the metabolism of microalgae.

The net monthly salary is between 1,500 € and 2,000 € for 36 months.

Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

Université de Nantes

Country

France

City

Nantes

Geofield

Where to apply

Website

https://www.abg.asso.fr/fr/candidatOffres/show/id_offre/121072

STATUS: EXPIRED