Postdoc Modelling of Reactive Oxygen Species Metabolism in Yeast Based on Novel Sensor Data

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Postdoc Modelling of Reactive Oxygen Species Metabolism in Yeast Based on Novel Sensor Data

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Job description

Offer DescriptionJoin us to create state-of-the-art metabolic and bioprocess models to capture the impact of reactive oxygen stress on process performance – and contribute to robust scale-up of bioprocesses.Job description
Among the many challenges faced by the growing bioeconomy, scaling biological systems to commercial volumes remains challenging. Well-calibrated dynamic bioprocess models can support this effort, but for these models to be representative of large-scale performance, measurements of the cellular response to various extracellular conditions are required. The team of Prof. Romana Schirhagl at the University medical center Groningen (UMCG) developed a novel sensing technique for intracellular quantification reactive oxygen species (RoS). RoS are involved in the cellular stress response as well as in aging, and therefore may correlate with bioprocess-relevant physiological traits, such as (by-) product formation. While this sensing technique was originally developed for medical applications, we are eager to explore application in bioprocesses. The bioprocess engineering section at TU Delft is collaborating with the group of Prof. Schirhagl to establish and evaluate this technology in a bioprocess context, and to use the data obtained from the sensor to develop state-of-the-art models for bioprocess assessment and scale-up based on free radical metabolism. The development and validation of such models forms the core of this postdoc project.In this 2 year postdoctoral project, you will provice a proof-of-concept for the construction of dynamic (empirical or mechanistic) models for bioprocess performance, based on the interplay between extracellular conditions, the intracellular reactive oxygen stress response and overall cell metabolism. Data from the novel RoS sensor, acquired under dynamic process conditions, serves for model calibration and validation. The activities in this project are all dry-lab (modelling) activities; the wet-lab data acquisition will be conducted at the UMCG in Groningen, with whom you will closely collaborate.The objective of this work is to study if we can capture:

• The ‘health’ of a bioreactor culture: can we relate process performance to shifts in RoS metabolism?
• Can we predict shifts in RoS following changes in operating/extracellular conditions, and can we use the model as an early indicator for a performance crash?
• Can we use model-based insights in RoS stress upon scale-up as an indicator of scale-up performance?

You will be part of the bioprocess engineering section at TU Delft, department of biotechnology, and will mainly work with Dr. ir. Cees Haringa (bioprocess modelling) and Dr. ir. Marieke Klijn (process analytical technology). Furthermore, you will have a strong interaction with the team at UMCG, who will conduct the underlying experimental work.We are looking for a postdoc with a strong background in the physiology of eukaryotic cell factories and modeling thereof, preferably in combination with a basic background in fermentation /bioprocess technology. To manage the different stakeholders in this project, obtain relevant experimental information from our partners, and to provide feedback for development and optimization of experimental procedures, we are looking for a candidate with good project management and communication skills.Job requirements

• Completed PhD in cell physiology, bioprocess engineering, or a similar discipline
• Expertise in modelling / programming is desired
• Strong collaborative and project management skills
• Prior expertise with (online) sensing and data analysis is beneficial, but not mandatory

TU Delft (Delft University of Technology)
Delft University of Technology is built on strong foundations. As creators of the world-famous Dutch waterworks and pioneers in biotech, TU Delft is a top international university combining science, engineering and design. It delivers world class results in education, research and innovation to address challenges in the areas of energy, climate, mobility, health and digital society. For generations, our engineers have proven to be entrepreneurial problem-solvers, both in business and in a social context.At TU Delft we embrace diversity as one of our core and we actively to be a university where you feel at home and can flourish. We value different perspectives and qualities. We believe this makes our work more innovative, the TU Delft community more vibrant and the world more just. Together, we imagine, invent and create solutions using technology to have a positive impact on a global scale. That is why we invite you to apply. Your application will receive fair consideration.Challenge. Change. Impact!Faculty Applied Sciences
With more than 1,100 employees, including 150 pioneering principal investigators, as well as a population of about 3,600 passionate students, the Faculty of Applied Sciences is an inspiring scientific ecosystem. Focusing on key enabling technologies, such as quantum- and nanotechnology, photonics, biotechnology, synthetic biology and materials for energy storage and conversion, our faculty aims to provide solutions to important problems of the 21st century. To that end, we educate innovative students in broad Bachelor’s and specialist Master’s programmes with a strong research component. Our scientists conduct ground-breaking fundamental and applied research in the fields of Life and Health Science & Technology, Nanoscience, Chemical Engineering, Radiation Science & Technology, and Engineering Physics. We are also training the next generation of high school teachers.Click to go to the website of the Faculty of Applied Sciences.Conditions of employment

• Duration of contract is 2 years.
• A job of 36-40 hours per week.

Salary and benefits are in accordance with the Collective Labour Agreement for Dutch Universities. The TU Delft offers a customisable compensation package, discounts on health insurance, and a monthly work costs contribution. Flexible work schedules can be arranged.For international applicants, TU Delft has the . This service provides information for new international employees to help you prepare the relocation and to settle in the Netherlands. The Coming to Delft Service offers a for partners and they organise events to expand your (social) network.Additional information
If you would like more information about this vacancy or the selection procedure, please contact Dr.ir. Cees Haringa, Assistant Professor Bioprocess Engineering, via .For more information about the application procedure, please contact our management assistant, mrs. Kawieta Ramautar, per email: .Application procedure
Are you interested in this vacancy? Please apply no later than 14 April 2025 via the application button and upload only the following documents:

• CV
• Motivational letter

You can address your application to Dr.ir. Cees Haringa, Assistant Professor Bioprocess Engineering.The application procedure may include an informal video call to talk about the motivation to apply for this position, this is before the formal interview with a larger committee. The formal inteview dates/times are fixed and will be performed on Monday 28 April at 9.00 – 10.30 and Tuesday at 9.00 – 13.30. Please keep these dates and times available. The envisioned starting date is June/July.Please note:

• You can apply online. We will not process applications sent by email and/or post.
• A pre-employment screening can be part of the selection procedure.
• For the final candidates, a knowledge security check will be part of the application procedure. For more information on this check, please consult Chapter 8 of the

. We carry out this check on the basis of legitimate interest. * Please do not contact us for unsolicited services.Where to apply WebsiteRequirementsAdditional InformationWebsite for additional job detailsWork Location(s)Number of offers available 1 Company/Institute TU Delft Country Netherlands City Delft Postal Code 2628CD Street Mekelweg 5 GeofieldContact CityDelft WebsiteStreetMekelweg 2 Postal Code2628 CDSTATUS: EXPIREDShare this page

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Location

Delft, Zuid-Holland

Job date

Thu, 13 Mar 2025 02:01:32 GMT

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